Osmotic concentration of urine in the dynamics of the development of alloxan-induced experimental diabetes

In order to clarify the peculiarities of osmotic concentration urine in dynamics development alloxan-induced experimental diabetes mellitus (DDM), studies were conducted on 54 sexually mature non-linear male white rats, which DDM was simulated by a single intraperitoneal injection alloxan solution (Alloxan monohydrate, "Acros Organics", Belgium) at dose 160 mg/kg body weight after previous 12-hour food deprivation with preserved access water. 10, 25, and 45 days introduction diabetogenic substance, 24 alloxan-diabetic as well 30 control (intact) animals, loading tap water volume 5% weight, collected for 2 hours, euthanasia performed decapitation under light ether anesthesia. blood samples, level glucose creatinine determined, evaluating water-induced 2-hour diuresis (in ml/100 g hours), clearance endogenous creatinine, osmotically free water, relative (tubular) reabsorption determined content glucose, urea, its excretion, including per 100 μl glomerular filtrate, osmolarity. The results study allow us state that duration is prolonged, pathogenetic significance hyperfiltration, determines speed fluid movement along tubule intensity absorption, weakens relation processes urine, yielding other factors, such osmolarity medulla kidneys, presence substances liquid distal segment collecting tubes, can change their tubes progresses. diabetes, addition include urea. Attempts analyze renal excretion urea revealed changes urinary are definitely correlated osmolality. Moreover, it be assumed transport disorders diabetic kidney occur earlier than ability excrete active general. A joint serve an early verifier tubulointerstitial damage. Nevertheless, increase reliability interpretation features we consider necessary nature electrolytes (sodium, potassium, calcium, ammonium).